CCR-10-3244.R1 miR-34a induces the down-regulation of both E2F1 and B-Myb oncogenes in leukemic cells
نویسندگان
چکیده
Giorgio Zauli, Rebecca Voltan, Maria Grazia di Iasio, Raffaella Bosco, Elisabetta Melloni, Maria Elena Sana, Paola Secchiero Institute for Maternal and Child Health, IRCCS “Burlo Garofolo”, Trieste, Italy; Department of Morphology and Embryology and LTTA Centre, University of Ferrara, Ferrara, Italy. §These author equally contributed to the work Corresponding author: Dr. Rebecca Voltan, Department of Morphology and Embryology and LTTA Centre, University of Ferrara, Via Fossato di Mortara 66, 44121 Ferrara, Italy. Tel: 39-0532455846; Fax: 39-0532-455950; E-mail: [email protected] Running title: miR34a down-regulates B-Myb expression
منابع مشابه
miR-34a induces the downregulation of both E2F1 and B-Myb oncogenes in leukemic cells.
PURPOSE To elucidate new molecular mechanisms able to downregulate the mRNA levels of key oncogenes, such as B-Myb and E2F1, in a therapeutic perspective. EXPERIMENTAL DESIGN B-Myb and E2F1 mRNA levels were evaluated in primary B chronic lymphocytic leukemia (B-CLL, n = 10) and acute myeloid leukemia (AML, n = 5) patient cells, in a variety of p53(wild-type) and p53(mutated/deleted) leukemic ...
متن کاملHuman Cancer Biology miR-34a Induces the Downregulation of Both E2F1 and B-Myb Oncogenes in Leukemic Cells
Purpose: To elucidate new molecular mechanisms able to downregulate the mRNA levels of key oncogenes, such as B-Myb and E2F1, in a therapeutic perspective. Experimental Design: B-Myb and E2F1mRNA levels were evaluated in primary B chronic lymphocytic leukemia (B-CLL, n1⁄4 10) and acute myeloid leukemia (AML, n1⁄4 5) patient cells, in a variety of p53 and p53 leukemic cell lines, as well as in p...
متن کاملmiR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53.
The role of miRNAs in regulating megakaryocyte differentiation was examined using bipotent K562 human leukemia cells. miR-34a is strongly up-regulated during phorbol ester-induced megakaryocyte differentiation, but not during hemin-induced erythrocyte differentiation. Enforced expression of miR-34a in K562 cells inhibits cell proliferation, induces cell-cycle arrest in G(1) phase, and promotes ...
متن کاملPLATELETS AND THROMBOPOIESIS miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53
TheroleofmiRNAs inregulatingmegakaryocyte differentiation was examined using bipotent K562 human leukemia cells. miR-34a is strongly up-regulated during phorbol ester–induced megakaryocyte differentiation, but not during hemin-induced erythrocyte differentiation. Enforced expression of miR-34a in K562 cells inhibits cell proliferation, induces cell-cycle arrest in G1 phase, and promotes megakar...
متن کاملTumor-suppressive miR-34a induces senescence-like growth arrest through modulation of the E2F pathway in human colon cancer cells.
Accumulating evidence suggests a role for microRNAs in human carcinogenesis as novel types of tumor suppressors or oncogenes. However, their precise biological role remains largely elusive. In the present study, we aimed to identify microRNA species involved in the regulation of cell proliferation. Using quantitative RT-PCR analysis, we demonstrated that miR-34a was highly up-regulated in a hum...
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تاریخ انتشار 2011